Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector

Abstract
A chimeric gene, consisting of the 5''-flanking region of a member of the Pisum sativum gene family encoding ribulose 1,5-bisphosphate carboxylase linked to the coding region of a bacterial chloramphenicol acetyltransferase gene, was introduced into the genome of N. tabacum using a Ti plasmid of Agrobacterium tumefaciens. The expression of the chimeric gene is light-inducible in chloroplast-containing transformed tissue.
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