A Passive Hemagglutination Test for Detecting Bovine Serum Albumin Antibodies

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Abstract
A microtiter passive hemagglutination test is described for detecting and titrating antibodies in chickens. Chicken erythrocytes are coated with antigen (bovine serum albumin = BSA) in the presence of 0.25% glutaraldehyde. Such sensitized erythrocytes are agglutinated in the presence of antibodies against BSA. The sensitivity and specificity of the test are not affected by salt concentrations in the range of 0.9-9%. This test measures the course of antibody response in chickens immunized with BSA.