Heteroduplex mobility assay of the D1/D2 region of the 26S rDNA for differentiation of Saccharomyces species

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Abstract
Aims: We present the HMA method for Saccharomyces differentiation using the PCR amplified D1/D2 26S rDNA. Methods and Results: This methodology is based on heteroduplex formation when two different DNAs are hybridized. We tested 11 type cultures of Saccharomyces, 27 different cultures of S. cerevisiae and four other ascomycetic genera. Conclusions: The method was capable of differentiating Saccharomyces species and was mainly very efficient for S. cerevisiae identification. HMA can probably be applied in other genera, where identification is sometimes difficult only by conventional traits, which are based on physiology and morphology. Significance and Impact of the Study: HMA provides a rapid and relatively simple molecular tool, contributing for yeast taxonomy.