Inhibition of ecdysteroid increase by diflubenzuron inTenebrio molitorpupae and compensation of diflubenzuron effect on cuticle secretion by 20-hydroxyecdysone
- 1 December 1984
- journal article
- research article
- Published by Taylor & Francis in International Journal of Invertebrate Reproduction and Development
- Vol. 7 (6) , 323-332
- https://doi.org/10.1080/01688170.1984.10510110
Abstract
Dipping of newly ecdysed Tenebrio molitor pupae in an acetone solution of diflubenzuron prevents most of them (73%) from carrying on further development. Such blocked animals cannot secrete adult cuticle and remain apolysed. Their ecdysteroid levels analysed by radioimmunoassay do not present any increase. However, injection of 20-hydroxyecdysone (2–10 μg) several days after diflubenzuron application allows the secretion of a new cuticle with an abnormal architecture but with a high content in N-acetyl-aminosugars as revealed by fluorescent wheat germ agglutinin.Keywords
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