Denitrification in Lake 227 During Summer Stratification

Abstract
In situ denitrification in Lake 227 was assayed by using 15N-labeled NO3 during summer stratification periods in 1973, 1974, and 1975. Dissolved oxygen and NO3 concentrations were the main factors controlling denitrification in the water column and in sediments. The epilimnetic sediment–water interface was a more significant site of natural denitrification when compared to the oxygen-limiting (dissolved oxygen less than 0.2 mg/L) thermocline and the anoxic hypolimnion. Epilimnetic sediment denitrification rates averaged about 15 mg N∙m−2∙d−1. Nearly all of the NO3 that mixed into the surficial sediments was denitrified. Alone, epilimnetic denitrification removed 1.4% of the NO3 added annually. Below the euphotic zone, sediments would provide an efficient sink for NO3-N without simultaneous initiation of eutrophication.Key words: denitrification, nitrogen sink, nitrate in lakes, experimental lakes, summer stratification

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