Cellular Fatty Acid Profile Distinguishes Burkholderia pseudomallei from Avirulent Burkholderia thailandensis
Open Access
- 1 October 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (10) , 4812-4814
- https://doi.org/10.1128/jcm.41.10.4812-4814.2003
Abstract
Burkholderia pseudomallei , the cause of melioidosis, can be distinguished from the closely related but nonpathogenic Burkholderia thailandensis by gas chromatography (GC) analysis of fatty acid derivatives. A 2-hydroxymyristic acid derivative (14:0 2OH) was present in 95% of B. pseudomallei isolates and no B. thailandensis isolates. GC mass spectrophotometry confirmed that 2-hydroxymyristic acid was present in B. pseudomallei . GC-fatty acid methyl ester analysis may be useful in distinguishing these two closely related species.Keywords
This publication has 15 references indexed in Scilit:
- Comparison of Rapid, Automated Ribotyping and DNA Macrorestriction Analysis ofBurkholderia pseudomalleiJournal of Clinical Microbiology, 2002
- Oxygen Requirement for the Biosynthesis of theS-2-Hydroxymyristate Moiety in Salmonella typhimurium Lipid APublished by Elsevier ,2000
- Characterization of the ybdT gene product of Bacillus subtilis: Novel fatty acid β‐hydroxylating cytochrome P450Lipids, 1999
- Dry-season outbreak of melioidosis in Western AustraliaThe Lancet, 1998
- Note: Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like speciesInternational Journal of Systematic and Evolutionary Microbiology, 1998
- Molecular Cloning and Expression of Fatty Acid α-Hydroxylase from Sphingomonas paucimobilisJournal of Biological Chemistry, 1997
- Ribotype differences between clinical and environmental isolates of Burkholderia pseudomalleiJournal of Medical Microbiology, 1997
- Isolation and identification of Burkholderia pseudomallei from soil using selective culture techniques and the polymerase chain reactionJournal of Applied Microbiology, 1997
- Cellular Lipid and Fatty Acid Compositions of Burkholderia pseudomallei Strains Isolated from Human and Environment in Viet NamMicrobiology and Immunology, 1995
- MELIOIDOSIS: A CASE-REPORTThe Lancet, 1964