Simple Detection of Fluorescent Stained IgM in Sucrose Gradients: Demonstration of Virus-Specific IgM

1 January 1974

journal article
Published by S. Karger AG in Intervirology

Vol. 3 (5-6) , 353-358
https://doi.org/10.1159/000149772

Abstract

Serum samples were mixed with appropriate amounts of fluorescein isothio-cyanate (FITC) at pH 9, then centrifuged on sucrose gradients. After separation, the FITC-conjugated IgM antibodies could be removed from the gradient under direct visual control. Staining of the sera facilitated the isolation and detection of IgM as demonstrated for rubella IgM-containing sera.

Keywords

IGM ANTIBODIES
FLUORESCENT STAINING
SUCROSE GRADIENTS
RUBELLA

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