Multiple isoforms of a protein kinase C inhibitor (KCIP‐1/14‐3‐3) from sheep brain
Open Access
- 1 June 1992
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 206 (2) , 453-461
- https://doi.org/10.1111/j.1432-1033.1992.tb16946.x
Abstract
A potent inhibitor of protein kinase C (PKC), inhibitor protein-1 (KCIP-1), isolated form sheep brain has been shown to consist of eight isoforms by reverse-phase HPLC. Direct protein sequence analysis has revealed these to be the same as those of 14-3-3 protein, described as an activator of tyrosine and tryptophan hydroxylases involved in neurotransmitter biosynthesis. The N-termini of KCIP-1 isoforms were shown to be acetylated, and secondary structure predictions revealed a high degree of α-helix with an amphipathic nature. KCIP-1 showed no inhibitory activity towards protein kinase M (the catalytic fragment of PKC) and had no effect on the activities of three other protein kinases, cAMP-dependent protein kinase, Ca2+/calmodulin-dependent protein kinase II and casein kinase 2. Four forms of KCIP-1 were shown to be substrates for PKC in vitro, but none were phosphorylated by the other protein kinases mentioned above.Keywords
This publication has 35 references indexed in Scilit:
- A plant homologue to mammalian brain 14‐3‐3 protein and protein kinase C inhibitorFEBS Letters, 1992
- Distinct forms of the protein kinase-dependent activator of tyrosine and tryptophan hydroxylasesJournal of Molecular Biology, 1991
- Involvement of activator protein in the activation of tryptophan hydroxylase by cAMP‐dependent protein kinaseFEBS Letters, 1990
- THE PROTEIN KINASE C FAMILY: HETEROGENEITY AND ITS IMPLICATIONSAnnual Review of Biochemistry, 1989
- A simple in situ cyanogen bromide cleavage method to obtain internal amino acid sequence of proteins electroblotted to polyvinyldifluoride membranesBiochemical and Biophysical Research Communications, 1988
- Primary structure of protein B from Pseudomonas putida, member of a new class of 2Fe‐2S ferredoxinsFEBS Letters, 1988
- K-252 compounds, novel and potent inhibitors of protein kinase C and cyclic nucleotide-dependent protein kinasesBiochemical and Biophysical Research Communications, 1986
- Staurosporine, a potent inhibitor of phospholipidCa++dependent protein kinaseBiochemical and Biophysical Research Communications, 1986
- Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteinsJournal of Molecular Biology, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970