Determination of Sugar in Plant Materials: Measurement of Unreduced Copper by Atomic Absorption Spectrometry

Abstract
An atomic absorption spectrophotometer was used for the quantitative analysis of reducing sugars by determining unreduced copper. Copper reduced in alkaline solution by sugar forms insoluble Cu2O. The Cu20 was centrifuged from the solution, and the unreduced copper in solution was measured with an atomic absorption spectrophotometer. The concentration of sugar present in test solutions was determined from a working curve prepared from known amounts of sugar.

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