Bryodin, a ribosome-inactivating protein from the roots of Bryonia dioica L. (white bryony)

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Abstract
1. Bryodin is a strongly basic (pI .gtoreq. 9.5) glycoprotein (neutral sugar content 6.3%) with Mr 30000, purified from the roots of Bryonia dioica (white bryony). 2. This protein inhibits protein synthesis by a rabbit reticulocyte lysate with an ID50 (concentration causing 50% inhibition) of 0.12 nM (3.6 ng/ml) and has much less effect on protein synthesis by whole cells, with ID50 values ranging from 46 nM to 2.27 .mu.M (1.4-67 .mu.g/ml). 3. Bryodin acts by inactivating ribosomes, with a less-than-equimolar ratio, which suggests a catalytic action 4. Bryodin decreases the number of local lesions induced by tobacco mosaic virus in the leaves of Nicotiana glutinosa. 5. From all its properties, bryodin can be considered to be a ribosome-inactivating protein, similar to those already known [reviews: Barbieri and Stirpe (1982) Cancer Surveys 1, 489-520; Stirpe and Barbieri (1986) FEBS Lett. 195, 1-8].