Etude de l'influence préalable de différentes enzymes amylolytiques sur la teneur en résidu NDF d'aliments du bétail

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Abstract
The initial VAN SOEST method (VAN SOEST and WINE, 1967) designed for roughages can lead to an overestimation of cell-wall constituent contents (estimated as NDF residues), especially in the case of starch rich feeds. To overcome this deficiency ROBERTSON and VAN SOEST (1977) suggested the use of a bacterial .alpha.-amylase. The aim of this study was to compare the influence of three amylolytic enzymes used at higher concentrations than those generally used, on NDF content of feedstuff samples, the extent of starch contamination of NDF residue and the possible effects (not of amylolytic origin) of these enzymes. Samples tested were very different in their starch content. The A 1278 amylase (Sigma Ltd) used at 45.degree. C allowed to extract almost all starch contained in the different samples, except from leguminosous seeds, as shown by starch content of NDF residue (Table 1). Results obtained with this enzyme were satisfactory and similar to those obtained with the A 6505 (Table 2). Measurement of starch content of NDF residue obtained with or without amylolytic treatment showed that enzymes possessed cellulasic and hemicellulasic activities (Table 3). This study allowed to develop a method of analysis of simple or compound feeds providing a solution to filtration difficulties related to starch content (fig. 1).

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