Inhibitory Effects of -Carotene on Proliferation of the Human Neuroblastoma Cell Line GOTO

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Abstract
α-Carotene inhibited the proliferation of the human neuroblastoma cell line GOTO in a dose- and time-dependent manner. In addition, it was about 10 times more inhibitory than β-carotene. Northern blot analysis indicated that α-carotene caused maximum suppression of the level of the N-myc messenger RNA of GOTO cells. This suppression occurred within 18 hours of α-carotene treatment, after which the level of the N-myc messenger RNA gradually recovered to the basal level. Analysis by flow cytometry indicated that when GOTO cells were exposed to α-carotene, they were arrested in the G0-G1 phase of their cell cycle. However, as the level of the N-myc messenger RNA was recovering, these cells resumed normal cycling. These results indicate that the reduction in the level of the N-myc messenger RNA caused by α-carotene is closely linked with G0-G1 arrest. [J Natl Cancer Inst 81:1649–1652, 1989]