EFFECTS OF RIBONUCLEASE AND DEOXYRIBONUCLEASE ON A MURINE LEUKEMIA VIRUS (RAUSCHER)

  • 1 January 1966
    • journal article
    • research article
    • Vol. 36  (3) , 465-+
Abstract
Plasma pellets and femoral bone marrow from BALB/cJ mice infected with the Rauscher leukemia virus were fixed, embedded, and sectioned. The thin sections were incubated in ribonuclease and deoxyribonuclease solutions, stained, and examined in the electron microscope. Specific attention was paid to the action of the nucleases on characteristic virus particles in the plasma preparations and on viruses being produced by the "budding" phenomenon in the femoral bone marrow. Ribonuclease solutions digested the nucleoids of the virus particles in the plasma preparations from mice infected with Rauscher leukemia virus. The nucleoid protion of the "budding" virus particles in bone marrow, and the connecting cytoplasm of the stalks were also digested by ribonuclease solutions. In addition, the outer coat of the "budding" particles was affected in a non-specific manner. The centers of the "budding particles in the bone marrow and the nucleoids of viruses in plasma preparations were not digested by deoxyribonuclease solutions. Influenza virus, a known ribonucleic acid (RNA) virus, was used as a control for nuclease activity. The nucleoids of influenza virus particles were digested by ribonuclease but not by deoxyribonuclease solutions. After coriphosphine staining of the plasma virus preparations, the fluorescence was quenched in preparations treated with ribonuclease, but did not appear to be diminished in those treated with deoxyribonuclease. This study suggests that infection of mice with Rauscher leukemia virus produces virus particles in plasma and "budding" particles in bone marrow, both of which contain RNA.