Regulation of bovine cervical secretion of prostaglandins and synthesis of cyclooxygenase by oxytocin

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Abstract
Prostaglandin E2 (PGE2) can cause softening of the bovine cervix at oestrus when receptors for oxytocin (OT) are maximally present, indicating a relationship between OT and PGE2 production. It was therefore determined whether OT can stimulate prostaglandin synthesis or induce cyclooxygenase expression in cervical external os segments obtained from pre-oestrous–oestrous cows. Tissues were minced and incubated (50–100 mg mL-1 6 h-1) in the presence of OT (10 ng mL-1), progesterone (P4)(5 ng mL-1) and/or indomethacin (5 µg mL-1). It was found that OT stimulated basal PGE2 (7·79 ± 1·22 ng 100 mg-1 , mean±s.e.m.; n = 6) in external os segments from pre-oestrous–oestrous cows (P < 0·03), whereas P4 and indomethacin inhibited basal and OT-stimulated PGE2 production (P < 0·05). Basal prostaglandin F2α (PGF2α ) production was minimal (

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