The Effect of Polyamines on Herpes Simplex Virus Type 1 DNA Polymerase Purified from Infected Baby Hamster Kidney Cells (BHK-21/C13)
- 1 August 1980
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 49 (2) , 397-400
- https://doi.org/10.1099/0022-1317-49-2-397
Abstract
The DNA polymerase whose synthesis is directed by the herpes simplex virus type 1 (HSV-1) DNA was purified 545-fold from BHK-21/C13 cells 16 h after infection with the virus. Spermidine and spermine stimulated the activity of the polymerase over the concentration range 0.5 mm to 2.5 mm. This effect was enhanced with increased concentrations of polyamine, maximum stimulation being threefold and fourfold for spermidine and spermine respectively. The diamine, putrescine, had little effect on the enzyme at the concentrations used (0.25 to 1.25 mm).This publication has 1 reference indexed in Scilit:
- Inhibition of polyamine accumulation and deoxyribonucleic acid synthesis in regenerating rat liverBiochemical Journal, 1976