Electrogeneration of a Poly(pyrrole)-NTA Chelator Film for a Reversible Oriented Immobilization of Histidine-Tagged Proteins
- 8 March 2005
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 127 (16) , 5752-5753
- https://doi.org/10.1021/ja050390v
Abstract
This contribution reports, for the first time, the synthesis and electropolymerization of a pyrrole N-substituted by a nitrilotriacetic acid acting as a chelating center of Cu2+. A step-by-step approach for protein immobilization was developed via the successive coordination of Cu2+ and histidine-tagged proteins. The self-assembly of histidine-tagged glucose oxidase led to the formation of a close-packed enzyme monolayer at the poly(pyrrole) surface, and the reversibility and reproducibility of this affinity process were demonstrated.Keywords
This publication has 7 references indexed in Scilit:
- Fabrication of Histidine-Tagged Fusion Protein Arrays for Surface Plasmon Resonance Imaging Studies of Protein−Protein and Protein−DNA InteractionsAnalytical Chemistry, 2003
- Factors governing the solubilization of phosphopeptides retained on ferric NTA IMAC beads and their analysis by MALDI TOFMSJournal of the American Society for Mass Spectrometry, 2002
- Layer-by-Layer Construction of Enzyme Multilayers on an Electrode for the Preparation of Glucose and Lactate Sensors: Elimination of Ascorbate Interference by Means of an Ascorbate Oxidase MultilayerAnalytical Chemistry, 1998
- Reversible Oriented Surface Immobilization of Functional Proteins on Oxide SurfacesAnalytical Chemistry, 1997
- A Self-Assembled Monolayer for the Binding and Study of Histidine-Tagged Proteins by Surface Plasmon ResonanceAnalytical Chemistry, 1996
- Synthesis and Characterization of Chelator-Lipids for Reversible Immobilization of Engineered Proteins at Self-Assembled Lipid InterfacesJournal of the American Chemical Society, 1994
- Electroconducting conjugated polymers: New sensitive matrices to build up chemical or electrochemical sensors. A reviewSensors and Actuators B: Chemical, 1992