ANTITUMOR-ACTIVITY OF AN IMMUNOTOXIN IN A NUDE-MOUSE MODEL OF HUMAN OVARIAN-CANCER

  • 1 March 1987
    • journal article
    • research article
    • Vol. 47  (5) , 1407-1410
Abstract
An immunotoxin composed of an antibody to the human transferrin receptor (454A12) and ricin A chain (RTA) was shown to inhibit the growth of NIH:OVCAR-3 tumors in a nude mouse model of human ovarian cancer. Inhibition of tumor growth by 454A12-RTA was related to the dose administered. The antitumor activity of the immunotoxin was blocked by coinjection of excess antibody with immunotoxin. An immunotoxin made using 454A12 and recombinant ricin A chain (rRTA) had an activity similar to that made with native RTA. The administration of 10 .mu.g or greater of the immunotoxin 454A12-RTA/rRTA had significant antitumor activity. The injection of 30 .mu.g of an irrelevant immunotoxin, MOPC21-RTA, or 30 to 500 .mu.g of the 454A12 antibody had no antitumor activity.