Displacement of sulfonamides from bovine serum albumin by nonsteroidal anti-inflammatory drugs.

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Abstract
The effects of 6 nonsteroidal anti-inflammatory drugs, phenylbutazone, flufenamic acid, salicylic acid, indomethacin, flurbiprofen and naproxen, on the binding of sulfonamides to bovine serum albumin were studied using the equilibrium dialysis method at pH 7.4 and 37.degree. C. Lineweaver-Burk plots suggested that the anti-inflammatory drugs inhibit the binding of sulfonamides competitively. Anti-inflammatory drugs could be ranked in the following order of diminishing ability to inhibit the binding of sulfonamides to bovine serum albumin: phenylbutazone, flufenamic acid, salicylic acid, indomethacin, flurbiprofen and naproxen; the inhibitory activities of the first 3 drugs were especially high. The competitive binding of sulfisoxazole and salicylic acid was studied in detail on the basis of a theoretical equation. The data for the inhibition of the binding of sulfisoxazole by salicylic acid satisfied the equation confirming that this theoretical equation is suitable to describe such competitive binding phenomena.