Targeting of non-lg sequences in place of the V segment by somatic hyper mutation
- 1 July 1995
- journal article
- Published by Springer Nature in Nature
- Vol. 376 (6537) , 225-229
- https://doi.org/10.1038/376225a0
Abstract
Affinity maturation of antibodies is characterized by localized hypermutation of the DNA around the V segment. Here we show, using mice containing single or multiple transgene constructs, that an immunoglobulin V kappa segment can be replaced by human beta-globin or prokaryotic neo or gpt genes without affecting the rate of hypermutation; the V gene itself is not necessary for recruiting hypermutation. The ability to target hypermutation to heterologous genes in vivo could find more general applications in biology.Keywords
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