Electrophoretic analysis of the molecular weight of murine mammary tumor virus RNA

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Abstract
Molecular weight determinations of native and subunit RNA of murine mammary tumor virus (MuMTV), a type B oncornavirus, were performed by polyacrylamide gel electrophoresis and compared with molecular weights of well-characterized avian cellular RNA and tobacco mosaic virus RNA. Extrapolations of semilog plots of the molecular weights of the standard RNA vs. relative electrophoretic mobilities and Ferguson plots showed that the subunit and native RNA of MuMTV possessed MW of 2.93 .times. 106 and 6.45 .times. 106, respectively. These data support the assumption that 2 subunit molecules comprise the native RNA of MuMTV.