Design and Use of Fluorescent Fusion Proteins in Cell Biology
Open Access
- 1 June 2005
- journal article
- unit
- Published by Wiley in Current Protocols in Cell Biology
- Vol. 27 (1) , 21.4.1-21.4.13
- https://doi.org/10.1002/0471143030.cb2104s27
Abstract
This unit describes strategies for designing functional fluorescent fusion protein constructs. Such constructs can be exploited as probes of cellular environments, protein dynamics, protein life histories, protein binding partners, and markers in living cells. The properties and uses of many currently available fluorescent proteins are discussed. In addition, alternative approaches and troubleshooting guidelines are provided.Keywords
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