Development of Beryllium Lymphocyte Transformation Tests in Chronic Beryllium Disease
- 1 January 1982
- journal article
- research article
- Published by S. Karger AG in International Archives of Allergy and Immunology
- Vol. 67 (2) , 175-180
- https://doi.org/10.1159/000233010
Abstract
We found that optimal conditions for the beryllium transformation test required the use of purified lymphocyte suspensions in 20% human serum, with beryllium sulphate at concentrations of 10––6 to 10––7M. Using these conditions, all 15 chronic beryllium patients tested gave a stimulation index of more than 2. Despite the above, 10––5 and 10––6M, BeSO4 inhibited spontaneous thymidine incorporation and lymphocyte transformation to PHA and PPD in normal subjects. Alternative methods, using whole blood, venous and thumb-prick samples, gave fewer and less reproducible positive results.Keywords
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