GUINEA PIG LYMPHOTOXIN AND COMPARISON OF VARIOUS ASSAY METHODS FOR CYTOTOXICITY

1 December 1972

journal article
research article
Published by Wolters Kluwer Health in Transplantation

Vol. 14 (6) , 771-776
https://doi.org/10.1097/00007890-197212000-00016

Abstract

SUMMARY The conditions for the production of lymphotoxin from guinea pig lymph node cells stimulated with Phaseolus vulgaris phytohemagglutinin (PHA) are described. A comparison of various assay methods of lymphotoxin, including the incorporation of isotopically labeled compounds, standard cell counting, and the release of isotope from ⁵¹Cr-labeled cells, was made. The method utilizing ¹⁴C-amino acid or ³H-inositol uptake was the most satisfactory in both sensitivity and accuracy. The advantages and disadvantages of other assay methods are also described.

Keywords

LYMPHOTOXIN
PIG
ISOTOPE
51CR
MADE
PHYTOHEMAGGLUTININ
NODE
STIMULATED
DISADVANTAGES

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