Differential Expression of Protein Kinase C Genes in Cultured Mast Cells Derived from Normal and Mast-Cell-Deficient Mice and Mast Cell Lines

Publisher Website
Google Scholar
Abstract
We investigated the expression of mRNA of protein kinase C ( PKC) isozymes (α, β, γ, δ, ε, ξ, and η) in cultured mast cells (CMC) derived from normal (+/+) mice, CMC derived from genetically mast-cell-deficient (W/W, Wv/Wv, and mi/mi) mice, and murine mast cell lines (IC2, MC9, and P-815) by Northern blotting. In +/+ CMC, abundant expression of PKCδ and moderate expression of PKCα and β was observed, while other PKCs (types γ, ε, ξ, and η) were not detected. In vivo expression of PKCδ was demonstrated in the skin by in situ hybridization. In mast cell lines, the expression pattern of PKC isozymes was similar to that of +/+ CMC, except that the expression of PKCη was detected in the IC2 cell line. The expression levels of PKCδ in CMC derived from c-kit-deficient mutants, W/W,Wv/Wv, and mi/mi, were lower than that of +/+ mice. These results indicate that PKCδ is the main isozyme in various types of murine mast cells and also suggest that the reduced level of PKCδ expression in mutant mice may be caused by a deficit in the signal transduction system through c-kit receptor.

This publication has 0 references indexed in Scilit: