Multiplex approaches in protein microarray technology
- 1 August 2005
- journal article
- review article
- Published by Taylor & Francis in Expert Review of Proteomics
- Vol. 2 (4) , 499-510
- https://doi.org/10.1586/14789450.2.4.499
Abstract
The success of genome sequencing projects has provided the basis for systematic analysis of protein function and has led to a shift from the description of single molecules to the characterization of complex samples. Such a task would not be possible without the provision of appropriate high-throughput technologies, such as protein microarray technology. In addition, the increasing number of samples necessitates the adaptation of such technologies to a multiplex format. This review will discuss protein microarray technology in the context of multiplex analysis and highlight its current prospects and limitations.Keywords
This publication has 85 references indexed in Scilit:
- Subnanoliter enzymatic assays on microarraysProteomics, 2005
- Protein microarrays: catching the proteomeMechanisms of Ageing and Development, 2005
- Readout of protein microarrays using intrinsic time resolved UV fluorescence for label‐free detectionProteomics, 2004
- Seeing Better through a MIST: Evaluation of Monoclonal Recombinant Antibody Fragments on MicroarraysAnalytical Chemistry, 2004
- Cell-Free Protein Expression and Functional Assay in Nanowell Chip FormatAnalytical Chemistry, 2004
- Microarrays to characterize protein interactions on a whole‐proteome scaleProteomics, 2003
- Dissecting DNA‐protein and protein‐protein interactions involved in bacterial transcriptional regulation by a sensitive protein array method combining a near‐infrared fluorescence detectionProteomics, 2003
- Protein analysis on a proteomic scaleNature, 2003
- Quantitative measurement of serum allergen-specific IgE on protein chipExperimental & Molecular Medicine, 2002
- Computational analysis of microarray dataNature Reviews Genetics, 2001