Photolabelling of a mitochondrially encoded subunit of NADH dehydrogenase with [3H]dihydrorotenone
Open Access
- 13 July 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 219 (1) , 108-112
- https://doi.org/10.1016/0014-5793(87)81200-0
Abstract
Mitochondrial NADH dehydrogenase from bovine heart was photolabelled with the inhibitor [3H]dihydrorotenone. A constituent of the hydrophobic domain of the enzyme of M r 33 000 was the major site of labelling. The identity of this protein with the mitochondrially encoded ND‐1 gene product was established by immunoblotting and immunoprecipitation with an antiserum raised to the expected C‐terminal sequence of the human ND‐1 gene product.Keywords
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