Quantitative Paper Chromatography of Fatty Acids. II. Some General Considerations on Direct Photometry.
- 1 January 1963
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 17 (1) , 187-190
- https://doi.org/10.3891/acta.chem.scand.17-0187
Abstract
Theoretical considerations together with experimental results indicate that letting the scanning window cover most of the width of the spots will render no significant bias to a chromatographie assay according to Kaufmann-Seher when the spot contents are kept below the 20 [mu]g limit for the validity of Beer''s law for every spot area element. It is recommended that the length of the scanning window should cover a little less than the largest visible width of the spot.Keywords
This publication has 1 reference indexed in Scilit:
- Direct Quantitation of Paper Chromatograms of Radioactivity from 14C Labelled Fatty Acids.Acta Chemica Scandinavica, 1961