Silage from tropical fish 3. Lipid behaviour

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Abstract
Acid silages were prepared from silverbelly (Leiognathus spp.) at 30°C with 3% (w/w) of 98% formic acid. Lipid oxidation takes place actively during ensilation as demonstrated by changes in fatty acid composition, TBA value and peroxide value. Lipid extraction, with chloroform: methanol or isopropanol, or the addition of an antioxidant before ensilation can limit the build up of lipid oxidation products. Lipid extraction also suppresses the autolysis of proteins in a defatted silage compared with a normal silage when measured by total soluble nitrogen or soluble NPN. The solubilization of collagen appears to be unaffected by lipid extraction perhaps reflecting the non‐enzymic nature of this process. The limited autolysis in defatted silages may be beneficial in restricting the release of free amino acids capable of reacting with lipid oxidation products resulting in a lower nutritional value for silage based diets.

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