Synthesis of Chloroplast Ribonucleic Acid in Chlamydomonas reinhardtii Toluene‐Treated Cells

Abstract
C. reinhardtii cells treated with toluene at 0.degree. C and 25.degree. C incorporate ribonucleoside triphosphates (NTP) into chloroplast RNA at 25.degree. C and also at 35.degree. C. The incorporation requires all 4 NTP and Mg2+, and is completely inhibited by DNase, RNase, actinomycin D (40 .mu.g/ml) and rifampicin (350 .mu.g/ml). However, the incorporation is almost totally insensitive to both .alpha.-amanitin and streptolydigin at 200 .mu.g/ml.