Number of genes and base composition of mitochondrial tRNA from Saccharomyces cerevistae
Open Access
- 1 June 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (6) , 831-838
- https://doi.org/10.1093/nar/2.6.831
Abstract
Increasing amounts of mitochondrial [32P] tRNA (4S fraction) were hybridized with mitochondrial DNA of Saccharomyces cerevisiae. At saturation, the calculated number of genes for 4S mitochondrial RNA was 20. Mitochondrial [32P] tRNA eluted from the hydrids obtained either with an excess of tRNA or an excess of DNA showed, after alkaline hydrolysis and chromatography, a G + C content of 28 and 35 p. cent respectively. This last value is similar to that found with the total 4S fraction. The odd nucleotides T (about IT per sequence), U, hU are present in mitochondrial tRNA. Some sequences may begin with pG.Keywords
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