Polyadenylate-protein complexes in resting and growing 3T3 cells
- 1 July 1979
- journal article
- research article
- Published by Wiley in Cell Biology International Reports
- Vol. 3 (4) , 373-381
- https://doi.org/10.1016/s0309-1651(79)80007-7
Abstract
The properties of the ribonuclease resistant cytoplasmic ribonucleoprotein particles were studied in contact-inhibited and serum induced proliferating 3T3 cells. The RNP particles were fractionated by oligo (dT)-cellulose chromatography and banded in CsSO4 gradients. The main RNP fraction, eluted with 25% formamide, contained the major ribonuclease resistant RNA sequences in both resting and growing cells. The protein component of this fraction had a molecular weight of about 72,000 in contact-inhibited cells and 81,000 in serum induced cells.Keywords
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