Effects of calcium ions on proteolytic processing of HIV‐1 gp160 precursor and on cell fusion
- 7 February 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 338 (3) , 281-284
- https://doi.org/10.1016/0014-5793(94)80284-x
Abstract
Complete activation of human immunodeficiency virus type 1 (HIV-1) requires the endoproteolytic cleavage by cellular protease of the envelope glycoprotein precursor (gp160) into the external glycoprotein gp120, and the transmembrane glycoprotein gp41. We report here the effect of depletion of cellular calcium ions on maturation of precursor gp160 and its concomitant effect on syncytium formation. We show that the cellular endoprotease activity responsible for gp160 maturation and the capacity for HIV-1 to induce syncytium formation are calcium-dependent. In addition, we show that endoproteolytic maturation is a key step in syncytium formation induced by HIV-1Keywords
This publication has 29 references indexed in Scilit:
- Inhibition of furin-mediated cleavage activation of HIV-1 glycoprotein gpl60Nature, 1992
- Mammalian subtilisins: The long-sought dibasic processing endoproteasesCell, 1991
- Endoproteolytic cleavage of gp160 is required for the activation of human immunodeficiency virusCell, 1988
- Functional Regions of the Envelope Glycoprotein of Human Immunodeficiency Virus Type 1Science, 1987
- pH-independent HIV entry into CD4-positive T cells via virus envelope fusion to the plasma membraneCell, 1987
- Role of the HTLV-III/LAV envelope in syncytium formation and cytopathicityNature, 1986
- Binding of HTLV-III/LAV to T4 + T Cells by a Complex of the 110K Viral Protein and the T4 MoleculeScience, 1986
- T-lymphocyte T4 molecule behaves as the receptor for human retrovirus LAVNature, 1984
- The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirusNature, 1984
- Detection, Isolation, and Continuous Production of Cytopathic Retroviruses (HTLV-III) from Patients with AIDS and Pre-AIDSScience, 1984