Ligation-anchored PCR unveils immune repertoire of TCR-beta from whole blood
Open Access
- 28 May 2015
- journal article
- research article
- Published by Springer Nature in BMC Biotechnology
- Vol. 15 (1) , 39
- https://doi.org/10.1186/s12896-015-0153-9
Abstract
As one of the genetic mechanisms for adaptive immunity, V(D)J recombination generates an enormous repertoire of T-cell receptors (TCRs). With the development of high-throughput sequencing techniques, systematic exploration of V(D)J recombination becomes possible. Multiplex PCR has been previously developed to assay immune repertoire; however, the use of primer pools leads to inherent biases in target amplification. In our study, we developed a “single-primerKeywords
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