Über Teilreaktionen der Photosynthetischen Sulfatreduktion in zellfreien Systemen aus Spinatchloroplasten und Chlorella / Enzyme Reactions Involved in Photosynthetic Sulfate-Reduction in Cell-free Systems of Spinach Chloroplasts and Chlorella

Open Access

1 February 1972

journal article
Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung B

Vol. 27 (2) , 183-192
https://doi.org/10.1515/znb-1972-0214

Abstract

Cellfree extracts from Chlorella, spinach leaves or spinach chloroplasts convert radioactive sulfate into a S-sulfocompound already in the dark. The reaction requires ATP, Mg^2⊕ ions and compounds with SH groups. The later are needed for activation of the enzym and as substrates and acceptors for the sulfogroup. The compound formed if glutathion is used has been identified as S-sulfoglutathion. Other products formed from sulfate and ATP in the dark by spinach or chlorella extracts are APS and PAPS. The formation of PAPS requires the presence of a SH-group. Both cellfree extracts transfer the sulfate group from APS and PAPS onto glutathion to form S-sulfoglutathion. No ATP is needed in this reaction, neither with PAPS nor with APS as substrate.

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