SEROLOGICAL PROPERTIES OF GAMMAA ANTIBODIES TO ESCHERICHIA COLI PRESENT IN HUMAN COLOSTRUM

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  • 1 January 1966
    • journal article
    • research article
    • Vol. 10  (6) , 517-+
Abstract
Antibodies against Esch. coli WF 96 and WF 61 present in human colostrum and serum were fractionated by DEAE-cellulose chromatography. Using the hemagglutination test it was found that the antibodies present in colostrum were recovered in the fraction containing the bulk of [gamma]A-globulin, whereas the antibodies present in serum were recovered in the fraction containing the bulk of [gamma] M-globulin. In the presence of human or guinea pig complement the antibodies present in colostrum did not lyse red cells coated with bacterial polysaccharides whereas the antibodies present in serum were lytic. When the properties of [gamma]A and [gamma]M antibodies were studied using a bacteriolytic system, it was observed that [gamma]A-globulin lysed bacteria only in the presence of both complement and lysozyme; in this respect [gamma] Abacterial antibodies behaved differently from [gamma]M antibodies which were bacteriolytic in the presence of complement alone, without lysozyme. The effect of treating [gamma]A and [gamma]M antibodies with 2-mercaptoethanol at neutral pH and of heating at 56[degree] was investigated.