CONSTRUCTION OF AN ENDOGLUCANOLYTIC BREWING YEAST STRAIN
- 12 November 1995
- journal article
- Published by Chartered Institute of Brewers and Distillers in Journal of the Institute of Brewing
- Vol. 101 (6) , 459-461
- https://doi.org/10.1002/j.2050-0416.1995.tb00884.x
Abstract
An endo‐β(1, 4)glucanase encoding gene from fungal origin has been expressed in a brewing yeast strain. The yeast transformation was carried out using a previously reported system based in the acquisition of cycloheximide resistance. The plasmid transferred to brewing yeast showed some changes in restriction pattern of the 2 μ portion after transformation while cycloheximide resistance marker and endoglucanase gene were not affected. The drug resistance phenotype showed by the recombinant yeast was highly stable in non‐selective conditions. The endoglucanase enzyme was detected in cell‐free culture medium and showed high activities in liquid and solid media.Keywords
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