Chemostat Culture of Escherichia coli K-12 Limited by the Activity of Alkaline Phosphatase
- 1 January 1975
- journal article
- research article
- Published by American Society for Microbiology in Applied Microbiology
- Vol. 30 (2) , 267-270
- https://doi.org/10.1128/aem.30.2.267-270.1975
Abstract
The growth-limiting reaction of a chemostat culture of Escherichia coli K-12 was the hydrolysis of β-glycerophosphate by alkaline phosphatase. The culture was buffered at pH 5.2 where alkaline phosphatase was unable to supply phosphate to the cell at a rate sufficient to sustain the maximum rate of growth. Alkaline phosphatase activity in this system is discussed in terms of the so-called Flip-Flop mechanism.Keywords
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