Inhibition of uptake of catecholamines by benzylamine derivatives

Publisher Website
Google Scholar
Abstract
Eight benzylamine analogs of bretylium were synthesized, incuding N-(2-chloroethyl)-N-methylbenzylamine (5), evaluated as inhibitors of accumulation of norepinephrine and dopamine in rat brain homogenates. All compounds gave an I50 value (concentration of inhibitor that causes 50% reduction in control accumulation) considerably lower against norpinephrine in cortex than against dopamine in striatum. High potency (low I50) and high specificity (preference for inhibition of norepinephrine transport compared to dopamine transport) are associated with a (2-cholorethyl) moiety, tertiary amino center, and ortho substitution of the aromatic function in the benzylamino group. In addition, 5 inhibited the uptake of norepinephrine in the rabbit aorta, indicating its effect against the uptake process in general. Cocaine protects against the effects of 5 in coincubation studies when compared to the appropriate controls, indicating that 5 acts at or close to the site of action of cocaine which is thought to be the uptake carrier site.