Quantification of HIV DNA in the brain by PCR: differences between fresh frozen and formalin fixed tissue.
Open Access
- 1 May 1996
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 49 (5) , 425-427
- https://doi.org/10.1136/jcp.49.5.425
Abstract
HIV-1 DNA extracted from frozen and formalin fixed brain tissue can be detected using PCR. This work has been extended by amplifying, using semiquantitative PCR, HIV DNA extracted from frontal lobe tissue of 16 patients with AIDS (eight positive and eight negative for p24 antigen). DNA was amplified using HIV-1 pol gene digoxigenin labelled primers and detected by chemiluminescence and densitometry. Cloned standards were amplified in parallel for quantification. HIV DNA levels detected in frozen tissue showed a correlation with p24 positivity and the severity of the histological diagnosis. This correlation was less clear in the formalin fixed material.Keywords
This publication has 8 references indexed in Scilit:
- PCR detection of HIV proviral DNA (gag) in the brains of patients with AIDS: comparison between results using fresh frozen and paraffin wax embedded specimens.Journal of Clinical Pathology, 1994
- Quantification of HIV by PCR in monocytes and lymphocytes in patients receiving antiviral treatment and low dose recombinant human granulocyte macrophage colony stimulating factor.Journal of Clinical Pathology, 1994
- Use of neuropathological tissue for molecular genetic studies: parameters affecting DNA extraction and polymerase chain reactionActa Neuropathologica, 1994
- Central nervous system damage produced by expression of the HIV-1 coat protein gpl20 in transgenic miceNature, 1994
- Human immunodeficiency virus type 1 infection of the nervous system: Pathogenetic mechanismsAnnals of Neurology, 1993
- HIV-Related Dementia: Pathology and Possible PathogenesisPublished by Springer Nature ,1993
- High levels of unintegrated HIV-1 DNA in brain tissue of AIDS dementia patientsNature, 1990