Mixed Supports in the Reversed-Phase Thin-Layer Chromatography of Amino Acids

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Abstract
Lipophilicity of amino acids was determined by reversed-phase thin-layer chromatography using silica, aluminium oxide, cellulose, diatomaceous earth and their mixtures as supports with water as eluent. To compare the retention behaviour of supports principal component analysis /PCA/ was applied. The potency order and the selectivity of support mixtures was calculated by the spectral map technique. Linear and logarithmic correlations were calculated between the first PCA loadings and the potency values as dependent variables and the composition of supports as independent variables. The first eigenvalue explained more than 90% of the total variance that is only one hidden factor influenced decisively the retention. On the basis of structural differences the retention strength of amino acids on support mixtures can not be explained adequately. The first principal component responsible for the 90% of change in the retention of amino acids is related to the logarithm of support composition that is the sorbents retain their original adsorptive character also after impregnation.