Monoclonal Antibodies Directed Against Human Fibroblast Interferon: Characterization and Functional Studies

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Abstract
Spleen cells from BALB/c mice, immunized with SDS-acrylamide gel purified human fibroblast interferon (HuIFN-β) were fused with mouse myeloma cells. Culture fluids from the resulting hybrid cells were screened for HuIFN-β specificity by the following tests: (1) a solid-phase RIA using partially purified HuIFN-β, (2) a protein-transfer RIA using electrophoretically resolved and immobilized HuIFN-β, and (3) a bioassay which tests residual HuIFN-β activity in the supernatant following immunoprecipitation. Six HuIFN-β-specific hybridomas were identified; two were capable of partially neutralizing HuIFN-β activity. All six antibodies bind to the β1-IFN polypeptide synthesized in E. coli cells containing a cloned β1-IFN DNA sequence. All six monoclonal antibodies were found to be IgG3/x.