High-pressure liquid chromatographic analysis of ceftazidime in serum and urine
Open Access
- 1 November 1983
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 24 (5) , 720-724
- https://doi.org/10.1128/aac.24.5.720
Abstract
A rapid and sensitive high-pressure liquid chromatographic procedure was developed for quantitative analysis of a new semisynthetic cephalosporin, ceftazidime, in serum and urine. A good linear relationship was established between peak height and the amount of ceftazidime injected over a concentration range of 1.9 to 30 micrograms/ml. Recovery was approximately 90% at concentrations of 3, 15, and 30 micrograms/ml. The method is specific for ceftazidime, with no interference noted from 11 other beta-lactam antibiotics tested. The assay is accurate, reproducible, and useful for pharmacokinetic studies in humans as demonstrated in two subjects.This publication has 5 references indexed in Scilit:
- Comparative activity in vitro of ceftazidime and nine other antibacterial agentsInfection, 1983
- Comparative antipseudomonal activity of some newer beta-lactam agentsAntimicrobial Agents and Chemotherapy, 1982
- Antibacterial activity and beta-lactamase stability of ceftazidime, an aminothiazolyl cephalosporin potentially active against Pseudomonas aeruginosaAntimicrobial Agents and Chemotherapy, 1982
- Comparison of in vitro activities of eight beta-lactamase-stable cephalosporins against beta-lactamase-producing gram-negative bacilliAntimicrobial Agents and Chemotherapy, 1981
- GR-20263: a new aminothiazolyl cephalosporin with high activity against Pseudomonas and EnterobacteriaceaeAntimicrobial Agents and Chemotherapy, 1980