CHANGES IN THE PHOSPHATE COMPOUNDS OF THE HUMAN RED BLOOD CELL DURING BLOOD BANK STORAGE*

Open Access
Publisher Website
Google Scholar
Abstract
Phosphorylated metabolic intermediates of the human red cell were analyzed by ion-exchange chromatography at approximately weekly intervals during 62 days of storage of blood in acid citrate dextrose (ACD) at 4[degree] C. There was a complete loss of fructose diphosphate and a reduction of adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (DPG) immediately upon mixing the blood with ACD, with a corresponding increase in Inorganic phosphate. Most of the DPG disappeared from the red cell during the first 2 weeks. The ATP concentration fell slowly during the same period and then more rapidly with a slower loss of adenosine diphosphate (ADP). The glucose diphosphate level remained fairly constant throughout the storage. Intracellular inorganic phosphate rose to a very high level at 2 weeks, with no further increase.