Diphospho-myo-inositol phosphates from Dictyostelium identified as d-6-diphospho-myo-inositol pentakisphosphate and d-5,6-bisdiphospho-myo-inositol tetrakisphosphate
- 15 February 1997
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 322 (1) , 31-33
- https://doi.org/10.1042/bj3220031
Abstract
Two diphospho-myo-inositol phosphates from Dictyostelium were recently investigated by two-dimensional 1H/31P NMR analysis and assigned to be either d-4-diphospho-myo-inositol pentakisphosphate (d-4-PP-InsP5) and d-4,5-bisdiphospho-myo-inositol tetrakisphosphate (d-4,5-bis-PP-InsP4) or their corresponding enantiomers d-6-PP-InsP5 and d-5,6-bis-PP-InsP4. In the present study the naturally occurring enantiomers were identified by using defined synthetic PP-InsP5 isomers as substrates for a partially purified PP-InsP5 5-kinase from Dictyostelium. This enzyme specifically phosphorylates the naturally occurring PP-InsP5 and the synthetic d-6-PP-InsP5, leading to d-5,6-bis-PP-InsP4. In contrast, neither d-4-PP-InsP5 nor d-1-PP-InsP5 or d-3-PP-InsP5 are converted by the enzyme.Keywords
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