Aminoacylation of Synthetic DNAs Corresponding to Escherichia coli Phenylalanine and Lysine tRNAs
- 1 July 1988
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 241 (4861) , 74-79
- https://doi.org/10.1126/science.2455342
Abstract
Synthetic DNA oligomers (tDNAs) corresponding to Escherichia coli tRNA(Phe) or tRNA(Lys) have been synthesized with either deoxythymidine (dT) or deoxyuridine (dU) substituted in the positions occupied by ribouridine or its derivatives. The tDNAs inhibited the aminoacylation of their respective tRNAs with their cognate amino acids, but not the aminoacylation of tRNA(Leu) with Leu. In the presence of aminoacyl-tRNA synthetase, species of both a tDNA(Phe) synthesized with a 3' terminal riboadenosine and a tDNA(Lys) containing only deoxynucleotides could be aminoacylated with the appropriate amino acids, although the Michaelis constant Km and observed maximal rate Vmax values for aminoacylation were increased by three- to fourfold and decreased by two- to threefold, respectively. The aminoacylation of synthetic tDNAs demonstrates that the ribose backbone of a tRNA is not absolutely required for tRNA aminoacylation.Keywords
This publication has 40 references indexed in Scilit:
- Base Modification and the Phosphoramidite ApproachNucleosides and Nucleotides, 1987
- Identification of transfer RNA suppressors in Escherichia coliJournal of Molecular Biology, 1984
- Hindered dialkylamino nucleoside phosphite reagents in the synthesis of two DNA 51-mersJournal of the American Chemical Society, 1983
- Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencingJournal of Molecular Biology, 1980
- Specificity of codon recognition by Escherichia coli tRNALeu isoaccepting species determined by protein synthesis in vitro directed by phage RNAJournal of Molecular Biology, 1979
- Proton nuclear magnetic resonance study of the effect of pH on tRNA structureBiochemistry, 1977
- Initial position of aminoacylation of individual Escherichia coli, yeast, and calf liver transfer RNAsBiochemistry, 1977
- Crystallographic refinement of yeast phenylalanine transfer RNA at 2·5Å resolutionJournal of Molecular Biology, 1976
- In vitro incorporation of 2′-deoxyadenosine and 3′-deoxyadenosine into yeast tRNAPhe using tRNA nucleotidyl transferase, and properties of tRNAPhe-C-C-2′dA and tRNAPhe-C-C-3′dABiochemical and Biophysical Research Communications, 1973
- The sequence of phenylalanine tRNA from E. coliFEBS Letters, 1969