High phylogenetic diversity of transconjugants carrying plasmid pJP4 in an activated sludge-derived microbial community
Open Access
- 1 June 2004
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 235 (2) , 215-219
- https://doi.org/10.1016/j.femsle.2004.04.038
Abstract
Transconjugants of plasmid pJP4, originating from an agar plate mating of a Pseudomonas putida donor with an activated sludge-derived microbial community, were isolated and identified by partial 16S rDNA sequencing. The transconjugant strains belonged to a variety of genera of the α-, β-, γβ- and γ-classes of the Proteobacteria, mostly to the families Rhizobiaceae and Comamonadaceae and the genus Stenotrophomonas. Only P. putida and Delftia spp. strains were able to grow on 2,4-D as the sole carbon source.Keywords
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