Characterization of thioredoxin y, a new type of thioredoxin identified in the genome of Chlamydomonas reinhardtii
Open Access
- 30 April 2003
- journal article
- Published by Wiley in FEBS Letters
- Vol. 543 (1-3) , 87-92
- https://doi.org/10.1016/s0014-5793(03)00416-2
Abstract
The sequencing of the Arabidopsis genome revealed a multiplicity of thioredoxins (TRX), ubiquitous protein disulfide oxido‐reductases. We have analyzed the TRX family in the genome of the unicellular green alga Chlamydomonas reinhardtii and identified eight different thioredoxins for which we have cloned and sequenced the corresponding cDNAs. One of these TRXs represents a new type that we named TRX y. This most probably chloroplastic TRX is highly conserved in photosynthetic organisms. The biochemical characterization of the recombinant protein shows that it exhibits a thermal stability profile and specificity toward target enzymes completely different from those of TRXs characterized so far.Keywords
This publication has 28 references indexed in Scilit:
- Chlamydomonas reinhardtii Genome Project. A Guide to the Generation and Use of the cDNA InformationPlant Physiology, 2003
- Divergent Light-, Ascorbate-, and Oxidative Stress-Dependent Regulation of Expression of the Peroxiredoxin Gene Family in ArabidopsisPlant Physiology, 2003
- Oxidation−Reduction and Activation Properties of Chloroplast Fructose 1,6-Bisphosphatase with Mutated Regulatory SiteBiochemistry, 2001
- Difference in the Mechanisms of the Cold and Heat Induced Unfolding of Thioredoxin h from Chlamydomonas reinhardtii: Spectroscopic and Calorimetric StudiesBiochemistry, 2000
- PLANT THIOREDOXIN SYSTEMS REVISITEDAnnual Review of Plant Biology, 2000
- Primary structure determinants of the pH- and temperature-dependent aggregation of thioredoxinBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 2000
- High-Level Expression of Recombinant Pea Chloroplast Fructose-1,6-Bisphosphatase and Mutagenesis of Its Regulatory SiteEuropean Journal of Biochemistry, 1995
- High‐Level Expression of Recombinant Pea Chloroplast Fructose‐1,6‐Bisphosphatase and Mutagenesis of Its Regulatory SiteEuropean Journal of Biochemistry, 1995
- Arabidopsis thaliana NAPHP Thioredoxin ReductaseJournal of Molecular Biology, 1994
- Human thioredoxin reactivity-structure/function relationshipBiochemical and Biophysical Research Communications, 1990