Radioimmunoassay of PCBs in Milk and Blood

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Abstract
A radioimmunoassay [RIA] was developed capable of determining Aroclor 1260 in milk at levels of 20-80 ppb and in blood from 2-16 ppb. The values obtained by RIA correlated well with those determined by GLC (r2 = 0.96 for milk and 0.99 for blood) but were lower. Antiserum was produced in rabbits and was specific for 2,2'',4,4'',5,5''-hexachlorobiphenyl. It cross-reacted with congeners and isomers in Aroclor 1254 and 1260 to the extent that a 20% decrease in binding was observed with 0.1 ng of either mixture. The method required preliminary cleanup of the extract on alumina and used 25% dimethyl sulfoxide in the assay medium to promote solubilization of the substrates.

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