Glucose-dependent insulinotropic polypeptide stimulated insulin release from a tumor-derived β-cell line (βTC3)

Publisher Website
Google Scholar
Abstract
The βTC3 tumor cell line was examined for the presence of functional glucose-dependent insulinotropic polypeptide (GIP) receptors. Increasing amounts of natural porcine GIP decreased the binding of HPLC-purified [125I]GIP to βTC3 cells in a concentration-dependent manner. Displacement of GIP was significant at concentrations as low as 500 pM, and the radioligand was fully displaced at 100 nM. GIP(1–30) produced a displacement of [125I]GIP comparable with that produced by GIP(1–42), and glucagon yielded 20% displacement at a concentration of 1 μM but was without effect at 100 nM. Incubation of βTC3 cells in the presence of glucose concentrations of 2–20 mM yielded a concentration-dependent stimulation of immunoreactive insulin (IRI) release. GIP and glucagon-like peptide-I(7–36) amide (tGLP-I) at concentrations of 1 nM or greater significantly stimulated IRI release in the presence of 2 mM glucose. The threshold glucose concentration for GIP-stimulated IRI release from βTC3 cells was 0.5 mM, and maximal potentiation of IRI release by GIP occurred at 5 mM glucose. Somatostatin significantly inhibited GIP-stimulated IRI release in the presence of 5 mM glucose. It is concluded that βTC3 cells have functional GIP receptors and may provide a useful model for the study of IRI secretion at the cellular level.Key words: insulin, βTC3, glucose-dependent insulinotropic polypeptide.

This publication has 0 references indexed in Scilit: