Production of Monoclonal Antibodies Specific to Major Allergens ofCryptomeria japonicaPollen
- 1 June 1992
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 11 (3) , 361-366
- https://doi.org/10.1089/hyb.1992.11.361
Abstract
Monoclonal antibodies (MAbs) directed against Cryptomeria japonica pollen antigen (CPAg) were prepared. CPAg was precipitated with these MAbs and both MAbs CPA7 (IgG2a) and CPA9 (IgG1) recognized two major glycoproteins (m=41kDa and 4 6 kDa) of CPAg. MAb CPA7 or CPA9 was coupled to CNBr activated sepharose and affinity purification of the major allergen of CPAg from the crude extract was performed. The affinity purified CPAg bound IgE antibody present in patients with Cedar pollinosis.Keywords
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